Metabolism of the preimplantation embryo: 40 years on
نویسندگان
چکیده
منابع مشابه
The Effect Of Vero Cells on Preimplantation Mouse Embryo in Cultur Medium
SUMMARY To study the effect of vero cells on preimplantation mouse embryos two groups of experiment were carried out. In experiment 1, pronuclear stage mouse embryos were co-cultured with Vero cells in RPMI medium and were examined under microscope for 4 days with 24h interval. The results showed co-cultured group developed better compared to control (87% to 80%, P<0.05) although, the rate ...
متن کاملThe Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
متن کاملpreimplantation mouse embryo
The role of membrane sterols in the compaction and subsequent development of the preimplantation mouse embryo was studied by incubating embryos in 7-ketocholesterol and other oxygenated sterols. These sterols have been shown to inhibit sterol synthesis and deplete membranes of cholesterol in a variety of other cell types. Compaction and subsequent blastocyst formation were normal when embryos w...
متن کاملReflections on the culture of the preimplantation embryo.
There has been a considerable improvement in the media available for the culture of preimplantation mouse embryos during the 40 years since mouse embryos were first cultured and successfully transferred to uterine foster mothers. Two new media, KSOM and mMTF, are becoming more commonly used. The history of the development of these media, including recent work on KSOM and mMTF, is reviewed. A ma...
متن کاملExperimental studies on the organization of the preimplantation mouse embryo
The interaction between mouse eggs conjoined in pairs of different ages and stages of development has been examined: 1. Forty-two per cent of chimaeras formed from 8-cell eggs paired with late morulae or early blastocysts produced morphologically normal, but large, blastocysts. 2. Fusion of pairs of vitally labelled and unlabelled eggs has shown that presumptive trophoblast derived from as late...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: REPRODUCTION
سال: 2012
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep-11-0484